Research Articles

King A.N., Fleming J.K., Knapik S.S., Visentin B., Wojciak J.M. & Huxford T. (2017).  High-affinity, pan-specific monoclonal antibodies that target cysteinyl leukotrienes and show efficacy in an acute model of colitis.  J. Lipid Res. 58 1386-1398. PubMed 5KVL

Geronimo I., Denning C.A., Rogers W.E., Othman T., Huxford T., Heidary D.K., Glazer E.C. & Payne C.M. (2016).  Effect of mutation and substrate binding on the stability of Cytochrome P450BM3 variants.  Biochemistry 55 3594-3606. PubMed 4ZFA 4ZF6 4ZFB 4ZF8

Hauenstein A.V., Rogers W.E., Shaul J.D., Huang D.-B., Ghosh G. & Huxford T. (2014). Probing kinase activation and substrate specificity with an engineered monomeric IKK2. Biochemistry 53 2064-2073. PubMed

Weerasinghe A.J., Amin S.A., Barker R.A., Othman T., Romano A.N., Parker Siburt C.J., Tisnado J., Lambert L.A., Huxford T., Carrano C.J. & Crumbliss A.L. (2013). Borate as a synergistic anion for Marinobacter algicola Ferric Binding Protein, FbpA: A role for boron in iron transport of marine life. J. Amer. Chem. Soc135 14504-14507. PubMed

Polley S., Huang D.-B., Hauenstein A.V., Fusco A.J., Zhong X., Vu D., Schröfelbauer B., Kim Y., Hoffmann A., Verma I.M., Ghosh G. & Huxford T. (2013). A structural basis for IκB Kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. PLoS Biol. 11 e1001581. PubMed 4E3C

Caldwell J.T.., Melkani G.C., Huxford T. & Bernstein S.I. (2012). Transgenic expression and purification of myosin isoforms using the Drosophila melaogaster indirect flight muscle system. Methods 56 25-32. PubMed

Fleming J.K., Wojciak J.M., Campbell, M.A. & Huxford T. (2011). Biochemical and structural characterization of lysophosphatidic acid binding by a humanized monoclonal antibody. J. Mol. Biol. 408 462-476. PubMed 3QCT 3QCU 3QCV

Lee C.F., Hauenstein A.V., Fleming J.K., Gasper W.C., Engelke V., Sankaran B., Bernstein S.I. & Huxford T. (2011). X-ray crystal structure of the UCS domain-containing UNC-45 myosin chaperone from Drosophila melanogasterStructure 19 397-408. PubMed 3NOW

Wojciak J.M., Zhu N., Schuerenberg K.T., Moreno K., Shestowsky W.S., Hiraiwa M., Sabbadini R. & Huxford T. (2009). The crystal structure of sphingosine-1-phosphate in complex with a Fab fragment reveals metal bridging of an antibody and its antigen. Proc. Natl. Acad. Sci. USA 106 17717-17722. PubMed 3I9G

Shaul J.D., Farina, A. & Huxford T. (2008). The human IKKβ subunit kinase domain displays CK2-like phosphorylation specificity. Biochem. Biophys. Res. Commun. 374 592-597. PubMed

Trinh D.V., Zhu N., Farhang G., Kim B.M. & Huxford T. (2008). The nuclear IκB protein IκBζ specifically binds NF-κB p50 homodimers and forms a ternary complex on κB DNA. J. Mol. Biol.379 122-135. PubMed

Bergqvist S., Croy C.H., Kjaergaard M., Huxford T., Ghosh G. & Komives E.A. (2006). Thermodynamics reveal that helix four in the NLS of NF-κB p65 anchors IκBα, forming a very stable complex. J. Mol. Biol. 360 421-434. PubMed

Hansen S.B., Sulzenbacher G., Huxford T., Marchot P., Taylor, P.W. & Bourne, Y. (2005). Structures of Aplysia AChBP complexes with agonists and antagonists reveal distinctive binding interfaces and conformations. EMBO J. 24 3635-3646. PubMed 2BYN 2BYP 2BYQ 2BYR 2BYS

Croy C.H., Bergqvist S., Huxford T., Ghosh G. & Komives E.A. (2004). Biophysical characterization of the free IκBα ankyrin repeat domain in solution. Protein Sci. 13 1767-1777. PubMed

Malek S., Huang D.-B., Chen Y., Huxford T., Ghosh S. & Ghosh G. (2003). X-ray crystal structure of an IκBβ/NF-κB p65 homodimer complex. J. Biol. Chem. 278 23094-23100. PubMed 1OY3 1K3Z

Huxford T., Mishler D., Phelps C.B., Chen Y., Sengchanthalangsy L.L., Reeves R., Hughes C.A., Komives E.A. & Ghosh G. (2002). Solvent exposed non-contacting amino acids play a critical role in NF-κB/IκBα complex formation. J. Mol. Biol. 324 587-597. PubMed 1MY5 1MY7

Malek S., Chen Y., Huxford T. & Ghosh G. (2001). IκBβ, but not IκBα, functions as a classical cytoplasmic inhibitor of NF-κB dimers by masking both NF-κB nuclear localization sequences in resting cells. J. Biol. Chem. 276 45225-45235. PubMed

Huxford T., Malek S., & Ghosh G. (2000). Preparation and crystallization of dynamic NF-κB/IκB complexes. J. Biol. Chem. 275 32800-32806. PubMed

Phelps C., Sengchanthalangsy L.L., Huxford T. & Ghosh G. (2000). Mechanism of IκBα binding to NF-κB dimers. J. Biol. Chem. 275 29840-29846. PubMed

Huxford T., Huang D.-B., Malek S. & Ghosh G. (1998). The crystal structure of the IκBα/NF-κB complex reveals mechanisms of NF-κB inactivation. Cell 95 759-770. PubMed

Malek S., Huxford T. & Ghosh G. (1998). IκBα functions through direct contacts with the nuclear localization signals and the DNA binding sequences of NF-κB. J. Biol. Chem. 273 25427-25435. PubMed 1IKN

Huang D.-B., Huxford T., Chen Y.-Q. & Ghosh G. (1997). The role of DNA in the mechanism of NF-κB dimer formation: crystal structures of the dimerization domains of the p50 and p65 subunits. Structure 5 1427-1436. PubMed 1BFS 1BFT

Lamb J.D., Huxford T. & Czirr K.B. (1996). Capillary electrophoresis of anions in an untreated polypropylene hollow fiber. J. Chromatogr. A 739 373-378. ScienceDirect


Review Articles

Huxford T., Hoffmann A. & Ghosh G. (2011). Understanding the logic of IκB:NF-κB regulation in structural terms. Curr. Top. Microbiol. Immunol. 349 1-24. PubMed

Huxford T. & Ghosh G. (2009). A structural guide to proteins of the NF-κB signaling module. Cold Spring Harb. Perspect. Biol. 1a000075. PubMed

Huxford T. & Ghosh G. (2005). Inhibition of transcription factor NF-κB activation by κB-Ras. Methods Enzymol. 407 527-534. PubMed

Ghosh G., Huang D.-B.& Huxford T. (2004) Molecular mimicry of the NF-κB DNA target site by a selected RNA aptamer. Curr. Opin. Struct. Biol. 14 21-27. PubMed

Huxford T. & Ghos ,G. (2002). IκB proteins. In, Wiley Encyclopedia of Molecular Medicine, John Wiley & Sons, Inc. 1712-1716. Wiley

Huxford T., Malek S. & Ghosh G. (1999). Structure and mechanism in NF-κB/IκB signaling. Cold Spring Harb. Symp. Quant. Biol. 64 533-540. PubMed

Ghosh G., Huang D.-B. & Huxford T. (1999). Structural insights into NF-κB/IκB signaling. Gene Therapy and Molecular Biology 4 75-82. Article


Book Chapters

Huxford T. (2013). X-ray crystallography. In, Brenner's Encyclopedia of Genetics, 2nd Edition. Edited by Stanley Maloy & Kelly Hughes. Academic Press, San Diego 366-368. ScienceDirect

Ghosh G., Huang D.-B. & Huxford T. (2010). Recognition of nucleic acids by transcription factor NF-κB DNA. In, Biophysics of DNA-Protein interactions:  From single molecules to biological systems.  Edited by Mark C. Williams & L. James Maher, III.  Springer-Verlag, New York 85-106. Amazon

Moorthy A.K., Huxford T. and Ghosh G. (2007) Structural aspects of NF-κB and IκB proteins In, Handbook of transcription factor NF-κB. Edited by Sankar Ghosh. Taylor and Francis CRC Press, Boca Raton, Florida 9-24. Amazon

Huxford T. & Ghosh G. (2006). Structural analysis of NF-κB and IκB proteins. In, NF-κB/Rel Transcription Factor Family. Edited by Hsiou-Chi Liou: Landes Bioscience, Georgetown, Texas 1-11. Landes Bioscience

Huxford T., Moorthy A.K. and Ghosh G. (2006) κB-Ras: a small GTPase that influences NF-κB signaling. In, RAS Family GTPases. Protein and Cell Regulation series, Volume 4. Edited by Channing J. Der. Springer Science, Dordecht, The Netherlands 341-352. Amazon

Huxford T. & Ghosh, G. (2003). The structural biology of NF-κB. In, Nuclear Factor κB: Regulation and Role in Disease. Edited by Beyaert R: Klüwer publishers, Netherlands 179-200. Amazon