Research Articles
Zhu N., Rogers W.E., Heidary D.K. & Huxford T. (2024). Structural and biochemical analyses of the nuclear IκBζ protein in complex with NF-κB p50 homodimer. Genes Dev. 38 528-535. Pubmed 9BOR
Mealka M., Sierra N.A., Avellaneda Matteo D., Albekioni E., Khoury R., Mai T., Conley B.M., Coleman N.J., Sabo K.A., Komives E.A., Bobkov A.A., Cooksy A.L., Silletti S., Schiffer J.M., Huxford T. & Sohl C.D. (2024). Active site remodeling in tumor-relevant IDH1 mutants drives distinct kinetic features and potential resistance mechanisms. Nat. Commun. 15 3785. Pubmed 8VHA 8VHB 8VHC 8VHD 8VHE 8VH9
Neal C.L., Kronert W.A., Camillo J.R.T., Suggs J.A., Huxford T. & Bernstein S.I. (2024). Aging-affiliated post-translational modifications of skeletal muscle myosin affect biochemical properties, myofibril structure, muscle function, and proteostasis. Aging Cell 23 e14134. PubMed
Zhu N., Mealka M., Mitchel S., Milani C., Acuña L.M., Rogers E., Lahana A.N. & Huxford T. (2023). X-ray crystallographic study of preferred spacing by the NF-κB p50 homodimer on κB DNA. Biomolecules 13 1310. PubMed 8TKL 8TKM 8TKN
Holmberg R., Robinson M., Gilbert S.F., Lujano-Olazaba O., Waters J.A., Kogan E., Velasquez C.L.R., Stevenson D., Cruz L.S., Alexander L.J., Lara J., Mu E.M., Camillo J.R., Bitler B.G., Huxford T. & House C.D. (2023). TWEAK-Fn14-RelB signaling cascade promotes stem cell-like features that contribute to post-chemotherapy ovarian cancer relapse. Mol. Cancer Res. 21 170-186. PubMed
Rana S., Kour S., Kizhake S., King H.M., Mallareddy J.R., Case A.J., Huxford T. & Natarajan A. (2022). Dimers of isatin derived α-methylene-γ-butyrolactone as potent anti-cancer agents. Bioorg. Med. Chem. Lett. 65 128713. PubMed
Ko M.S., Cohen S.N., Polley S., Mahata S.K., Biswas T., Huxford T. & Ghosh G. (2022). Regulatory subunit NEMO promotes polyubiquitin-dependent induction of NF-κB through a targetable second interaction with upstream activator IKK2. J. Biol. Chem. 298 101864. PubMed
Kour S., Rana S., Kizhake S., Lagundžin D., Klinkebiel D., Mallareddy J.R., Huxford T., Woods N.T. & Natarajan A. (2022). Stapling proteins in the RELA complex inhibits TNFα-induced nuclear translocation of RELA. RSC Chem. Biol. 3 32-36. PubMed
Napoleon J.V., Singh S., Rana S., Bendjennat M., Kumar V., Kizhake S., Palermo N.Y., Ouelette M.M., Huxford T. & Natarajan A. (2021). Small molecule binding to inhibitor of nuclear factor kappa-B kinase subunit beta in an ATP non-competitive manner. Chem. Commun. 57 4678-4681. PubMed
Ko M.S., Biswas T., Mulero M.C., Bobkov A.A., Ghosh G. & Huxford T. (2020). Structurally plastic NEMO and oligomerization prone IKK2 subunits define the behavior of human IKK2:NEMO complexes in solution. Biochim. Biophys. Acta Proteins Proteom. 1868 140526. PubMed
Farokhi E., Fleming J.K., Erasmus M.F., Ward A.D., Wu Y., Gutierrez M.G., Wojciak J.M. & Huxford T. (2020). Ion binding properties of a naturally occurring metalloantibody. Antibodies 9 10. PubMed 6VRT
Caldwell J.T., Mermelstein D.J., Walker R.C., Bernstein S.I. & Huxford T. (2020). X-ray crystallographic and molecular dynamic analyses of Drosophila melanogaster embryonic muscle myosin define domains responsible for isoform-specific properties. J. Mol. Biol. 432 427-447. PubMed 5W1A
Maniaci B., Lipper C.H., Anipindi L.D., Erlandsen H., Cole J.L., Stec B., Huxford T. & Love J.J. (2019). Design of high-affinity metal-controlled protein dimers. Biochemistry 58 2199-2207. PubMed 6NL6 6NL7 6NL8 6NL9 6NLA 6NLB
Mulero M.C., Shahabi S., Ko M.S., Schiffer J.M., Huang D.-B., Wang V.Y.-F., Amaro R.E., Huxford T. & Ghosh G. (2018). Protein cofactors are essential for high-affinity DNA binding by the Nuclear Factor κB RelA subunit. Biochemistry 57 2943-2957. PubMed
King A.N., Fleming J.K., Knapik S.S., Visentin B., Wojciak J.M. & Huxford T. (2017). High-affinity, pan-specific monoclonal antibodies that target cysteinyl leukotrienes and show efficacy in an acute model of colitis. J. Lipid Res. 58 1386-1398. PubMed 5KVL
Geronimo I., Denning C.A., Rogers W.E., Othman T., Huxford T., Heidary D.K., Glazer E.C. & Payne C.M. (2016). Effect of mutation and substrate binding on the stability of Cytochrome P450BM3 variants. Biochemistry 55 3594-3606. PubMed 4ZFA 4ZF6 4ZFB 4ZF8
Hauenstein A.V., Rogers W.E., Shaul J.D., Huang D.-B., Ghosh G. & Huxford T. (2014). Probing kinase activation and substrate specificity with an engineered monomeric IKK2. Biochemistry 53 2064-2073. PubMed
Weerasinghe A.J., Amin S.A., Barker R.A., Othman T., Romano A.N., Parker Siburt C.J., Tisnado J., Lambert L.A., Huxford T., Carrano C.J. & Crumbliss A.L. (2013). Borate as a synergistic anion for Marinobacter algicola Ferric Binding Protein, FbpA: A role for boron in iron transport of marine life. J. Amer. Chem. Soc. 135 14504-14507. PubMed
Polley S., Huang D.-B., Hauenstein A.V., Fusco A.J., Zhong X., Vu D., Schröfelbauer B., Kim Y., Hoffmann A., Verma I.M., Ghosh G. & Huxford T. (2013). A structural basis for IκB Kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. PLoS Biol. 11 e1001581. PubMed 4E3C
Caldwell J.T.., Melkani G.C., Huxford T. & Bernstein S.I. (2012). Transgenic expression and purification of myosin isoforms using the Drosophila melaogaster indirect flight muscle system. Methods 56 25-32. PubMed
Fleming J.K., Wojciak J.M., Campbell, M.A. & Huxford T. (2011). Biochemical and structural characterization of lysophosphatidic acid binding by a humanized monoclonal antibody. J. Mol. Biol. 408 462-476. PubMed 3QCT 3QCU 3QCV
Lee C.F., Hauenstein A.V., Fleming J.K., Gasper W.C., Engelke V., Sankaran B., Bernstein S.I. & Huxford T. (2011). X-ray crystal structure of the UCS domain-containing UNC-45 myosin chaperone from Drosophila melanogaster. Structure 19 397-408. PubMed 3NOW
Wojciak J.M., Zhu N., Schuerenberg K.T., Moreno K., Shestowsky W.S., Hiraiwa M., Sabbadini R. & Huxford T. (2009). The crystal structure of sphingosine-1-phosphate in complex with a Fab fragment reveals metal bridging of an antibody and its antigen. Proc. Natl. Acad. Sci. USA 106 17717-17722. PubMed 3I9G
Shaul J.D., Farina, A. & Huxford T. (2008). The human IKKβ subunit kinase domain displays CK2-like phosphorylation specificity. Biochem. Biophys. Res. Commun. 374 592-597. PubMed
Trinh D.V., Zhu N., Farhang G., Kim B.M. & Huxford T. (2008). The nuclear IκB protein IκBζ specifically binds NF-κB p50 homodimers and forms a ternary complex on κB DNA. J. Mol. Biol.379 122-135. PubMed
Bergqvist S., Croy C.H., Kjaergaard M., Huxford T., Ghosh G. & Komives E.A. (2006). Thermodynamics reveal that helix four in the NLS of NF-κB p65 anchors IκBα, forming a very stable complex. J. Mol. Biol. 360 421-434. PubMed
Hansen S.B., Sulzenbacher G., Huxford T., Marchot P., Taylor, P.W. & Bourne, Y. (2005). Structures of Aplysia AChBP complexes with agonists and antagonists reveal distinctive binding interfaces and conformations. EMBO J. 24 3635-3646. PubMed 2BYN 2BYP 2BYQ 2BYR 2BYS
Croy C.H., Bergqvist S., Huxford T., Ghosh G. & Komives E.A. (2004). Biophysical characterization of the free IκBα ankyrin repeat domain in solution. Protein Sci. 13 1767-1777. PubMed
Malek S., Huang D.-B., Chen Y., Huxford T., Ghosh S. & Ghosh G. (2003). X-ray crystal structure of an IκBβ/NF-κB p65 homodimer complex. J. Biol. Chem. 278 23094-23100. PubMed 1OY3 1K3Z
Huxford T., Mishler D., Phelps C.B., Chen Y., Sengchanthalangsy L.L., Reeves R., Hughes C.A., Komives E.A. & Ghosh G. (2002). Solvent exposed non-contacting amino acids play a critical role in NF-κB/IκBα complex formation. J. Mol. Biol. 324 587-597. PubMed 1MY5 1MY7
Malek S., Chen Y., Huxford T. & Ghosh G. (2001). IκBβ, but not IκBα, functions as a classical cytoplasmic inhibitor of NF-κB dimers by masking both NF-κB nuclear localization sequences in resting cells. J. Biol. Chem. 276 45225-45235. PubMed
Huxford T., Malek S., & Ghosh G. (2000). Preparation and crystallization of dynamic NF-κB/IκB complexes. J. Biol. Chem. 275 32800-32806. PubMed
Phelps C., Sengchanthalangsy L.L., Huxford T. & Ghosh G. (2000). Mechanism of IκBα binding to NF-κB dimers. J. Biol. Chem. 275 29840-29846. PubMed
Huxford T., Huang D.-B., Malek S. & Ghosh G. (1998). The crystal structure of the IκBα/NF-κB complex reveals mechanisms of NF-κB inactivation. Cell 95 759-770. PubMed
Malek S., Huxford T. & Ghosh G. (1998). IκBα functions through direct contacts with the nuclear localization signals and the DNA binding sequences of NF-κB. J. Biol. Chem. 273 25427-25435. PubMed 1IKN
Huang D.-B., Huxford T., Chen Y.-Q. & Ghosh G. (1997). The role of DNA in the mechanism of NF-κB dimer formation: crystal structures of the dimerization domains of the p50 and p65 subunits. Structure 5 1427-1436. PubMed 1BFS 1BFT
Lamb J.D., Huxford T. & Czirr K.B. (1996). Capillary electrophoresis of anions in an untreated polypropylene hollow fiber. J. Chromatogr. A 739 373-378. ScienceDirect
Review Articles
Mulero M.C., Wang V.Y.-F., Huxford T. & Ghosh G. (2019). Genome reading by the NF-κB transcription factors. Nucleic Acids Res. 47 9967-9989. PubMed
Huxford T., Hoffmann A. & Ghosh G. (2011). Understanding the logic of IκB:NF-κB regulation in structural terms. Curr. Top. Microbiol. Immunol. 349 1-24. PubMed
Huxford T. & Ghosh G. (2009). A structural guide to proteins of the NF-κB signaling module. Cold Spring Harb. Perspect. Biol. 1a000075. PubMed
Huxford T. & Ghosh G. (2005). Inhibition of transcription factor NF-κB activation by κB-Ras. Methods Enzymol. 407 527-534. PubMed
Ghosh G., Huang D.-B.& Huxford T. (2004) Molecular mimicry of the NF-κB DNA target site by a selected RNA aptamer. Curr. Opin. Struct. Biol. 14 21-27. PubMed
Huxford T. & Ghosh G. (2002). IκB proteins. In, Wiley Encyclopedia of Molecular Medicine, John Wiley & Sons, Inc. 1712-1716. Wiley
Huxford T., Malek S. & Ghosh G. (1999). Structure and mechanism in NF-κB/IκB signaling. Cold Spring Harb. Symp. Quant. Biol. 64 533-540. PubMed
Ghosh G., Huang D.-B. & Huxford T. (1999). Structural insights into NF-κB/IκB signaling. Gene Therapy and Molecular Biology 4 75-82. Article
Book Chapters
Mulero M.C., Huxford T. & Ghosh G. (2019). NF-κB, IκB, and IKK: Integral components of immune cell signaling. Adv. Exp. Med. Biol. 1172 207-226. PubMed
Huxford T. (2013). X-ray crystallography. In, Brenner's Encyclopedia of Genetics, 2nd Edition. Edited by Stanley Maloy & Kelly Hughes. Academic Press, San Diego 366-368. ScienceDirect
Ghosh G., Huang D.-B. & Huxford T. (2010). Recognition of nucleic acids by transcription factor NF-κB DNA. In, Biophysics of DNA-Protein interactions: From single molecules to biological systems. Edited by Mark C. Williams & L. James Maher, III. Springer-Verlag, New York 85-106. Amazon
Moorthy A.K., Huxford T. and Ghosh G. (2007) Structural aspects of NF-κB and IκB proteins In, Handbook of transcription factor NF-κB. Edited by Sankar Ghosh. Taylor and Francis CRC Press, Boca Raton, Florida 9-24. Amazon
Huxford T. & Ghosh G. (2006). Structural analysis of NF-κB and IκB proteins. In, NF-κB/Rel Transcription Factor Family. Edited by Hsiou-Chi Liou: Landes Bioscience, Georgetown, Texas 1-11. Landes Bioscience
Huxford T., Moorthy A.K. and Ghosh G. (2006) κB-Ras: a small GTPase that influences NF-κB signaling. In, RAS Family GTPases. Protein and Cell Regulation series, Volume 4. Edited by Channing J. Der. Springer Science, Dordecht, The Netherlands 341-352. Amazon
Huxford T. & Ghosh, G. (2003). The structural biology of NF-κB. In, Nuclear Factor κB: Regulation and Role in Disease. Edited by Beyaert R: Klüwer publishers, Netherlands 179-200. Amazon
